HILIC es el nombre abreviado de cromatografía de interacción hidrofílica y se desarrolló como una alternativa a la cromatografía de fase inversa para compuestos altamente polares. Inertsil HILIC es la columna que se une químicamente con el grupo diol y proporciona una excelente forma de pico para compuestos neutros y básicos. En modo HILIC, generalmente cuando se eleva la concentración orgánica, muestra una fuerte retención. Además, puede obtener un análisis más estable agregando un solvente básico al eluato, como acetato de amonio.
Recursos de columnas HILIC:
Columns | Features | USD Code | Particle Size (µm) | Pore Size (nm) | Surface Area (m2/g) | Carbon Loading (%) | Recommended pH range |
InertSustain Amide | First choice column for HILIC mode. | L68 | 3, 5 | 10 | 350 | 15 | 2 - 8.5 |
Inertsil Amide | Effective when the retention of high polar compounds is further strengthened. | L68 | 3, 5 | 10 | 450 | 18 | 2 - 7.5 |
Inertsil HILIC | Effective when the overall retention is to be reduced or when the separation pattern is to be changed. | L20 | 3, 5 | 10 | 450 | 20 | 2 - 7.5 |
InertSustain NH2 | First choice column for sugar analysis. | L8 | 3, 5 | 10 | 350 | 7 | 2 - 7.5 |
Inertsil NH2 | Effective for intensifying retention in sugar analysis | L8 | 3, 5 | 10 | 450 | 8 | 2 - 7.5 |
As an alternative to reversed-phase chromatography, Hydrophilic Interaction Chromatography (HILIC) was developed for analyses of highly polar compounds. The retention in HILIC mode usually increases with increasing concentration of an organic compound. Moreover, the stability is improved by adding a basic solvent (such as ammonium acetate) to the eluent.