MonoSpin ProA

Marca: MonoSpin

MonoSpin ProA es una columna de centrifugación con proteína A funcionalizada en un monolito de sílice para [¿para qué sirve ProA?]. La sílice monolítica es una rápida purificación de anticuerpos. La sílice se modifica con un polímero hidrófilo y luego se inmoviliza con proteína A o proteína G para evitar la adsorción de proteínas, lo que da como resultado una purificación rápida y una alta recuperación de anticuerpos.

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Overview

Purification of IgG Using MonoSpin ProA and MonoSpin ProG in only 5 min.

Sample can be neutralized and maintained in stable condition by putting neutral solution in tube when recovering anti-body.

As shown below, the anti-body concentrations were determined quantitatively from medium of CHO cells. The purified antibodies show very less impurities by the results from electrophoresis.

MonoSpin ProA ProG spin columns purification

Higher efficiency sorbent

The monolithic silica phase used in MonoSpin columns is state-of-the-art in stationary phase technology allowing high recovery and adsorption and desorption performance. The structure of the silica allows for less obstruction of the binding proteins compared to conventional particle based phases allowing for much higher efficiency of separation.

MonoSpin ProA ProG silica monolith vs. standard stationary phases

More than 90% Recovery Rate

MonoSpin ProA can achieve a 90% recovery rate of IgG using only 100 µL of elution buffer compared other other brands requiring 400 µL of buffer while only achieving a recovery rate of 70%.

Reproducible Performance in 96 Well Plate format

MonoSpin ProA's high manufacturing standards allows for uniform performance across wells in a 96 well plate format making MonoSpin ProA ideal for batch process.

Absorbance of MonoSpin ProA across wells. Sample volume 150 µL, elution volume 150 µL, recovery rate 90% (CV 3.1%), and IgG concentration 1.3 mg/mL.