MonoSpin ProG

Marca: MonoSpin

MonoSpin ProA es una columna de centrifugación funcionalizada con proteína G para la purificación de anticuerpos. La proteína G es una proteína derivada de bacterias que se une a las regiones Fab y Fc de las inmunoglobulinas. El esqueleto monolítico de sílice se modifica especialmente con un polímero hidrófilo y luego se inmoviliza con proteína G, lo que proporciona una purificación rápida y una alta recuperación de anticuerpos.

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Overview

Purification of IgG Using MonoSpin ProA and MonoSpin ProG in only 5 min.

Sample can be neutralized and maintained in stable condition by putting neutral solution in tube when recovering anti-body.

As shown below, the anti-body concentrations were determined quantitatively from medium of CHO cells. The purified antibodies show very less impurities by the results from electrophoresis.

MonoSpin ProA ProG spin columns purification

High recovery of Antibodies

Both MonoSpin ProA and MonoSpin ProG have 90% recovery (max 0.4 mg MonoSpin Pro A, max 0.3 mg MonoSpin ProG). MonoSpin ProG enables higher recovery and purification of IgG3 over MonoSpin ProA.

MonoSpin ProA ProG spin columns Relation between amount of IgG addiction and collected antibodies

Relation between amount of IgG addition and collection of antibodies. MonoSpin ProA (left), MonoSpin ProG (right)

Higher efficiency sorbent

The monolithic silica phase used in MonoSpin columns is state-of-the-art in stationary phase technology allowing high recovery and adsorption and desorption performance. The structure of the silica allows for less obstruction of the binding proteins compared to conventional particle based phases allowing for much higher efficiency of separation.

MonoSpin ProA ProG silica monolith vs. standard stationary phases

Removal of Preservatives in anti-body solutions

MonoSpin ProA/ProG enables you to remove proteins such as BSA and Gelatin in anti-body solutions without dilution.