The data on the right shows the superior performance of Titansphere TiO over another competitor brand. HeLa Cell Lysate which consists mainly of non-phosphorylated peptides, was used as sample. Titansphere TiO shows exceptional selectivity - almost 90 % of the bound peptides were phosphopeptides, and excellent capacity for total phosphopeptide binding. While the competitive brand shows more selectivity for non-posphorelated peptides.

Sample : HeLa Cell Lysate, Sample volume : 50 µg,
Titansphere TiO volume : 1 mg

The graph on the right shows the difference between Titansphere TiO and the normal IMAC enrichment method. Using Arabidopsis cell extract. Titansphere TiO provides substantially higher total peak area and a much higher number of non-phosphorelated peptides.

Sample : Arabidopsis Cell Extract, Sample volume : 100 µg,
Titansphere TiO volume : 1 mg